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KMID : 0387720090200030220
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Korean Journal of Blood Transfusion
2009 Volume.20 No. 3 p.220 ~ p.226
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An Experience with Unexpected Antibody Screening Tests using a Panel That Included Dia Cells in Koreans
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Yun Seung-Gyu
Yoo Byong-Joon
Yang Jin-Hyuk
Lim Chae-Seung
Kim Young-Kee
Lee Kap-No
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Abstract
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Background:In Korea, a screening panel of cells from abroad without Dia positive cells has been commonly used when a patient has an unexpected antibody screening test. It has been reported that Dia occurs with a frequency of 6.14 to 14.5% among Koreans. However, the current popular antibody screening panels contain no Dia positive cells. In this study, we evaluate the clinical usefulness of the Dia Cell Panel (Diagnostic Grifols, Barcelona, Spain) for Koreans.
Methods :A total of 3,372 pretransfusion samples were employed for unexpected antibody screening testing using panels of cells by the DG Gel microtube column agglutination system, including additional Dia cells (Diagnostic Grifols, Barcelona, Spain). The positive cases in this system were confirmed again with DiaMed Dia antigen positive panel cells (DiaMed Ag, Cresssier, Morat, Switzerland) and this was followed by sequence- based Diego genotyping.
Results :The positive detection rate of an unexpected antibody screening test using SeraScan Diana I and II was 1.07% (36/3372), and seven samples were reactive (1＋∼2＋) with the SeraScan Dia panel cells (0.21%). However, among the 5 available genotyped samples, two cases were typed as Di(a?b＋).
Conclusion :Even though there is discrepancy between the genotype and the two antibody screening kits, the addition of Dia positive cells as unexpected antibody screening panel cells is recommended. (Korean J Blood Transfus 2009;20:220-226)
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KEYWORD
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Anti-Dia, Dia antigen, Antibody screening
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